István Horváth Dr. Doctor of Biology Doctorof Medicine (Doctor of Biology) has devoted his life to cholesterol research aquarter of a century. He wrote his doctoral dissertation and doctoral thesis onDoctoral Biology. These studies were organically linked to the discovery ofanti-cholesterol antibodies and, on this basis, to find a vaccine suitable forrestoring cholesterol metabolism.
【霍尔瓦特伊斯特万】 MTA医学生物学博士（生物学博士），毕生致力于胆固醇的研究近1/4世纪。他撰写博士论文和博士论文。这些研究与抗胆固醇抗体的发现相关，并在此基础上发明一种适合恢复胆固醇代谢的疫苗。
When did you decide to deal withcholesterol research?
你什么时候决定开始研究胆固醇的？
In LIFE people often do not do what theydecided in their childhood. I was doing this as well. It was quite acoincidence that led me to start cholesterol research. For a long time, I wasdriving the Serology Department at the Ghost Department of Skin-Enemy. We werethe designated institute that verified the uncertain cases that occurred duringthe diagnosis of syphilis. He said that one of them had syphilis and the otherhad been exempted from having syphilis despite being positive in serologicalscreening. Research into the cause of non-syphilis but serological positivecauses led to a previously unknown antibody to cholesterol-relatedanti-cholesterol antibodies.
在生活中，人们常常做的事情不是他们童年时决定的事。我也是这样的。这是一个巧合促使我开始胆固醇研究。很长一段时间，我在皮肤研究所血清学科。我们是指定的机构，验证梅毒诊断过程中发生的不确定病例。他们中有一个得了梅毒，另一个已经没有梅毒了，尽管血清学筛查是阳性的。研究没有梅毒但血清是阳性的原因，让我发现前未知的与胆固醇相关的抗胆固醇抗体。
What was this antibody?
什么是抗体？
This is a cholesterol-specificimmunoglobulin. The production of specific immunoglobulins, antibodies,antigens. Being a cholesterol antigen may be a surprise to me because fordecades I have taught physicians and specialists that cholesterol is not anantigen. To my surprise this antibody responded specifically to it. Then, youare interested in the role of anti-cholesterol antibody in your body.
这是一种胆固醇特异性免疫球蛋白。特异性免疫球蛋白、抗体、抗原的产品。作为一个胆固醇抗原可能让我感到惊讶，因为几十年来，我教医生和专家，胆固醇不是抗原。令我惊讶的是这种抗体对它特别有反应。然后，你对体内抗胆固醇抗体的作用感兴趣。
At first I thought it was a role intriggering atherosclerosis. To address this question, it was necessary toproduce an antigen that could be used to increase the antibody production. Whenwe got there that we could boost the experimental animal, the production ofthis antibody was just the opposite. That is, it has an important role to playin preventing atherosclerosis, and even its re-development.
起初我认为它是引发动脉粥样硬化的一个角色。为了解决这个问题，有必要产生一种可以用来增加抗体产生的抗原。当我们到达那里，我们可以刺激实验动物，这种抗体的生产正好相反。也就是说，它在预防动脉粥样硬化甚至是再发展方面起着重要的作用。
When we started looking closely at theantibody, we discovered that it has a role in cholesterol metabolism. To ensurehealthy cholesterol metabolism, an adequate amount of cholesterol antibody isneeded.
当我们开始仔细研究抗体时，我们发现它在胆固醇代谢中起作用。为了确保健康的胆固醇代谢，需要足够数量的胆固醇抗体。
Where did these research go?
这些研究去哪里了？
They went to the National Institute ofDermatology. We got stunned about it, just about syphilis serology, we did notlook for it, we accidentally found it. Just like when you find a big amber onthe sea shore.
他们去了国立皮肤病研究所。我们对此感到震惊，关于梅毒血清学，我们没有寻找它，我们偶然发现了它。就像你在海边发现一个大琥珀一样。
This "amber" when we found it wasso new and contradicted all the knowledge that had been taught cholesterolearlier. When I visited the director and told him what a wonderful, completelynew discovery he was unable to accept. Indeed, it is not possible to do so, soI should know that.
这个“琥珀”，当我们发现它是如此新，并反驳所有的知识，已经教胆固醇早。当我去拜访主任，告诉他一个奇妙的、全新的发现时，他无法接受。事实上，这样做是不可能的，所以我应该知道。
According to them, as the discovery wasborn, has the struggle for it begun?
据他们说，随着发现的诞生，它的斗争开始了吗？
This was the first wall, becausecholesterol is a very important building material in our body and apre-material for important compounds. It plays a primary role in many areas. Itis made up of animal, human cell membranes. True, it does not have much to do.In every animal body, it occurs in the cell membrane, if it is very small, butthere. Using a questionnaire: what is the brick wall? Brick! - was the answer.The few. It is necessary for the mortar to connect. There must be cholesterolas a "mortar" if there is little to be in the cell membrane becausewithout that there can be no healthy membrane.
这是第一道墙，因为胆固醇是人体内非常重要的建筑材料，也是重要化合物的原料。它在许多领域起着主要作用。它是由动物、人体细胞膜组成的。是的，它没有太多的事情要做。在每一个动物身上，它都发生在细胞膜中，如果它很小，但在那里。用问卷调查：砖墙是什么？砖！-答案是很少。是必需的连接用的砂浆。必须要有胆固醇做砂浆。如果细胞膜中，就没有健康的细胞膜膜。
What were the stages of the finalformulation of the product?
产品研发的最后阶段是怎么样的？
We have discovered that there is ananti-cholesterol antibody.
我们已经发现了ACHA胆固醇抗体的存在
Acceptance and acceptance of this antibodywas very difficult since we have previously argued that cholesterol is not anantigen. How to develop antibodies against it if it is not an antigen.
接受和公认存在这个抗体是非常困难的，因为我们一直认为胆固醇不是抗原，那么如果不是抗原，如何产生抗体？
When it became apparent that cholesterolcould be an antigenic property, we began to research the production ofantigen-bearing cholesterol. Meanwhile, a method had to be developed toroutinely perform the antibody detection.
当发现胆固醇可能是一种抗原特性时，我们开始研究含抗原胆固醇的生产。同时，还必须开发一种常规的抗体检测方法。
Then we asked what this antibody was for?Because nothing in nature is just to be.
我们想抗体是什么？自然界有没有？
We developed an immunization protocol thatwas used to optimize antibody production. Several attempts have been made toconfirm the increase in antibody production, its intensity and itsadjuvant-related influence.
我们研发了优化了抗体的生产的免疫方案，几次实验都确认抗体的增加、强度和佐剂的影响关系
Observations were made on the interactionwith the antitoxins of bacteria in the body.
观测结果表明在体内一种人工细菌的抗毒素的相互作用的结果
When we had the diagnostic and immunizationmethod in our hands, we could begin researching what kind of pharmacology theanesthetist had in the living organism. At first it was clear that he wasacting in conjunction with cholesterol-related physiological processes.
当诊断和免疫方法已经发现，我们开始研究什么样药理作用在什么样的生物体内。起初，胆固醇相关的生理过程是很清晰
We used methods to induce atherosclerosis.These were rather time-consuming tasks because such an experiment lasted for 19to 25 weeks. Persistent work has yielded the result. It has become clear thatthe antibody has a clear effect on preventing and reversing atherosclerosis.
我们用方法诱导动动物产生脉粥样硬化。这是相关费时的任务，因为要19-25周才可以，持续的工作取的的成果。很明显，抗体对阻止和逆转动脉粥样硬化有明显的作用
We could not go to the task to clarify theimpact of reproduction on biological processes. Therefore, artificiallyfertilized animals have shown that their embryo defense function and thefertilization enhancing effect of increased antibody levels.
我们不能去做这项工作来阐明生殖对生物过程的影响。因此，人工受精的动物已经显示出它们的胚胎防御功能和增强抗体水平的受精作用。
【霍尔瓦特伊斯特万】 MTA医学生物学博士（生物学博士），毕生致力于胆固醇的研究近1/4世纪。他撰写博士论文和博士论文。这些研究与抗胆固醇抗体的发现相关，并在此基础上发明一种适合恢复胆固醇代谢的疫苗。
When did you decide to deal withcholesterol research?
你什么时候决定开始研究胆固醇的？
In LIFE people often do not do what theydecided in their childhood. I was doing this as well. It was quite acoincidence that led me to start cholesterol research. For a long time, I wasdriving the Serology Department at the Ghost Department of Skin-Enemy. We werethe designated institute that verified the uncertain cases that occurred duringthe diagnosis of syphilis. He said that one of them had syphilis and the otherhad been exempted from having syphilis despite being positive in serologicalscreening. Research into the cause of non-syphilis but serological positivecauses led to a previously unknown antibody to cholesterol-relatedanti-cholesterol antibodies.
在生活中，人们常常做的事情不是他们童年时决定的事。我也是这样的。这是一个巧合促使我开始胆固醇研究。很长一段时间，我在皮肤研究所血清学科。我们是指定的机构，验证梅毒诊断过程中发生的不确定病例。他们中有一个得了梅毒，另一个已经没有梅毒了，尽管血清学筛查是阳性的。研究没有梅毒但血清是阳性的原因，让我发现前未知的与胆固醇相关的抗胆固醇抗体。
What was this antibody?
什么是抗体？
This is a cholesterol-specificimmunoglobulin. The production of specific immunoglobulins, antibodies,antigens. Being a cholesterol antigen may be a surprise to me because fordecades I have taught physicians and specialists that cholesterol is not anantigen. To my surprise this antibody responded specifically to it. Then, youare interested in the role of anti-cholesterol antibody in your body.
这是一种胆固醇特异性免疫球蛋白。特异性免疫球蛋白、抗体、抗原的产品。作为一个胆固醇抗原可能让我感到惊讶，因为几十年来，我教医生和专家，胆固醇不是抗原。令我惊讶的是这种抗体对它特别有反应。然后，你对体内抗胆固醇抗体的作用感兴趣。
At first I thought it was a role intriggering atherosclerosis. To address this question, it was necessary toproduce an antigen that could be used to increase the antibody production. Whenwe got there that we could boost the experimental animal, the production ofthis antibody was just the opposite. That is, it has an important role to playin preventing atherosclerosis, and even its re-development.
起初我认为它是引发动脉粥样硬化的一个角色。为了解决这个问题，有必要产生一种可以用来增加抗体产生的抗原。当我们到达那里，我们可以刺激实验动物，这种抗体的生产正好相反。也就是说，它在预防动脉粥样硬化甚至是再发展方面起着重要的作用。
When we started looking closely at theantibody, we discovered that it has a role in cholesterol metabolism. To ensurehealthy cholesterol metabolism, an adequate amount of cholesterol antibody isneeded.
当我们开始仔细研究抗体时，我们发现它在胆固醇代谢中起作用。为了确保健康的胆固醇代谢，需要足够数量的胆固醇抗体。
Where did these research go?
这些研究去哪里了？
They went to the National Institute ofDermatology. We got stunned about it, just about syphilis serology, we did notlook for it, we accidentally found it. Just like when you find a big amber onthe sea shore.
他们去了国立皮肤病研究所。我们对此感到震惊，关于梅毒血清学，我们没有寻找它，我们偶然发现了它。就像你在海边发现一个大琥珀一样。
This "amber" when we found it wasso new and contradicted all the knowledge that had been taught cholesterolearlier. When I visited the director and told him what a wonderful, completelynew discovery he was unable to accept. Indeed, it is not possible to do so, soI should know that.
这个“琥珀”，当我们发现它是如此新，并反驳所有的知识，已经教胆固醇早。当我去拜访主任，告诉他一个奇妙的、全新的发现时，他无法接受。事实上，这样做是不可能的，所以我应该知道。
According to them, as the discovery wasborn, has the struggle for it begun?
据他们说，随着发现的诞生，它的斗争开始了吗？
This was the first wall, becausecholesterol is a very important building material in our body and apre-material for important compounds. It plays a primary role in many areas. Itis made up of animal, human cell membranes. True, it does not have much to do.In every animal body, it occurs in the cell membrane, if it is very small, butthere. Using a questionnaire: what is the brick wall? Brick! - was the answer.The few. It is necessary for the mortar to connect. There must be cholesterolas a "mortar" if there is little to be in the cell membrane becausewithout that there can be no healthy membrane.
这是第一道墙，因为胆固醇是人体内非常重要的建筑材料，也是重要化合物的原料。它在许多领域起着主要作用。它是由动物、人体细胞膜组成的。是的，它没有太多的事情要做。在每一个动物身上，它都发生在细胞膜中，如果它很小，但在那里。用问卷调查：砖墙是什么？砖！-答案是很少。是必需的连接用的砂浆。必须要有胆固醇做砂浆。如果细胞膜中，就没有健康的细胞膜膜。
What were the stages of the finalformulation of the product?
产品研发的最后阶段是怎么样的？
We have discovered that there is ananti-cholesterol antibody.
我们已经发现了ACHA胆固醇抗体的存在
Acceptance and acceptance of this antibodywas very difficult since we have previously argued that cholesterol is not anantigen. How to develop antibodies against it if it is not an antigen.
接受和公认存在这个抗体是非常困难的，因为我们一直认为胆固醇不是抗原，那么如果不是抗原，如何产生抗体？
When it became apparent that cholesterolcould be an antigenic property, we began to research the production ofantigen-bearing cholesterol. Meanwhile, a method had to be developed toroutinely perform the antibody detection.
当发现胆固醇可能是一种抗原特性时，我们开始研究含抗原胆固醇的生产。同时，还必须开发一种常规的抗体检测方法。
Then we asked what this antibody was for?Because nothing in nature is just to be.
我们想抗体是什么？自然界有没有？
We developed an immunization protocol thatwas used to optimize antibody production. Several attempts have been made toconfirm the increase in antibody production, its intensity and itsadjuvant-related influence.
我们研发了优化了抗体的生产的免疫方案，几次实验都确认抗体的增加、强度和佐剂的影响关系
Observations were made on the interactionwith the antitoxins of bacteria in the body.
观测结果表明在体内一种人工细菌的抗毒素的相互作用的结果
When we had the diagnostic and immunizationmethod in our hands, we could begin researching what kind of pharmacology theanesthetist had in the living organism. At first it was clear that he wasacting in conjunction with cholesterol-related physiological processes.
当诊断和免疫方法已经发现，我们开始研究什么样药理作用在什么样的生物体内。起初，胆固醇相关的生理过程是很清晰
We used methods to induce atherosclerosis.These were rather time-consuming tasks because such an experiment lasted for 19to 25 weeks. Persistent work has yielded the result. It has become clear thatthe antibody has a clear effect on preventing and reversing atherosclerosis.
我们用方法诱导动动物产生脉粥样硬化。这是相关费时的任务，因为要19-25周才可以，持续的工作取的的成果。很明显，抗体对阻止和逆转动脉粥样硬化有明显的作用
We could not go to the task to clarify theimpact of reproduction on biological processes. Therefore, artificiallyfertilized animals have shown that their embryo defense function and thefertilization enhancing effect of increased antibody levels.
我们不能去做这项工作来阐明生殖对生物过程的影响。因此，人工受精的动物已经显示出它们的胚胎防御功能和增强抗体水平的受精作用。
