All 16 Rune Combos and when you can unlock them
1 - 5f-4e-3d-2c-2b (after 600)
2 - 5b-4c-3d-2e-1f (after 700)
3 - 1b-2c-3d-2e-1f (after 800)
4 - 2b-2c-2d-2e-2f (after 900)
5 - 1b-1c-2d-3e-5f (after 1000)
Unlocked after shadow blade all at once (~lvl 1041)
6 - 3c-4d-5e-4f-3b
7 - 5f-5e-5d-5c-5b
8 - 3b-2d-1f-2c-3e
9 - 2d-3c-4e-3b-2f
10 - 5e-1b-3f-2c-4d
Available in Shadow Challenge only
11 - 4b-4c-2d-2e-2f (after 600)
12 - 1b-4c-1d-4e-1f (after 600)
13 - 5f-2e-3d-2c-5b (after shadow blade ~1041)
14 - 4b-1c-4d-1e-4f (after shadow blade ~1041)
15 - 3f-3e-4d-3c-3b (after 1500)
16 - 2b-1c-5d-1e-2f (after 1500)
1 - 5f-4e-3d-2c-2b (after 600)
2 - 5b-4c-3d-2e-1f (after 700)
3 - 1b-2c-3d-2e-1f (after 800)
4 - 2b-2c-2d-2e-2f (after 900)
5 - 1b-1c-2d-3e-5f (after 1000)
Unlocked after shadow blade all at once (~lvl 1041)
6 - 3c-4d-5e-4f-3b
7 - 5f-5e-5d-5c-5b
8 - 3b-2d-1f-2c-3e
9 - 2d-3c-4e-3b-2f
10 - 5e-1b-3f-2c-4d
Available in Shadow Challenge only
11 - 4b-4c-2d-2e-2f (after 600)
12 - 1b-4c-1d-4e-1f (after 600)
13 - 5f-2e-3d-2c-5b (after shadow blade ~1041)
14 - 4b-1c-4d-1e-4f (after shadow blade ~1041)
15 - 3f-3e-4d-3c-3b (after 1500)
16 - 2b-1c-5d-1e-2f (after 1500)